Antibacterial Activity Of Honey Thesis

Antibacterial Activity Of Honey Thesis-53
manuka honey) and preferred for wound management, although historically local honeys have been used.The main aim of this study was to investigate the mechanisms of antibacterial action of manuka honey.Staphylococci were more susceptible to all honeys than Pseudomonas aeruginosa and Escherichia coli.

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The antibacterial activity of honey can be divided into hydrogen peroxide and non-hydrogen peroxide-derived activity.

This later type of activity is characteristic of honeys from Australasia (e.g.

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Clinical strains of both bacteria showed different time-kill profiles to type strains.

The methodology used for MIC determination was found to affect to the results obtained.The antibacterial activity of manuka honey has previously been claimed to be due to hydrogen peroxide production and not to a non-peroxide source of activity.A study of free radical production and antioxidant potential demonstrated that manuka honey did not produce any hydroxyl radicals via the Fenton reaction. It was also observed that even free radical-producing honeys were able to quench radical production in vitro.There is an urgent need for new effective antimicrobial agents since acquired resistance of bacteria to currently available agents is increasing.The antimicrobial activity of Mono-floral Agastache honey produced from Australian grown Agastache rugosa was compared with the activity of commercially available honeys derived from Leptospermum species and with Jarrah honey for activity against clinical and non-clinical strains of Staphylococcus aureus (methicillin-susceptible and methicillin-resistant strains), Pseudomonas aeruginosa, and Escherichia coli.No honey-resistant ram-positive bacteria were recovered, but Gramnegative bacteria were found to be able to become phenotypically resistant to manuka honey.Electron microscopy showed that honey inflicted physical damages in both types of cells, and in Gram-positive bacteria led to an increase in the proportion of population of cells with a complete septum.In conclusion this study has demonstrated that the non-peroxide activity of manuka honey is not exclusive to Australasia honeys, that it is not derived from hydrogen peroxide generation and may have a microbial origin.Furthermore the action of manuka honey on Gram-negative bacteria seems to be more physical than in Gram positive where it appears to interfere with the cell physiology, perhaps by stopping the cell cycle before cytokinesis.By continuing to use this site, you consent to the use of cookies.We use cookies to offer you a better experience, personalize content, tailor advertising, provide social media features, and better understand the use of our services.

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